Identification and Characterisation of Potential Probiotic Lactic Acid Bacteria Extracted from Pig Faeces

Given that probiotics always have host-homologous and strain-specific effects on the hosts, lactic acid bacteria extracted identified from porcine specimens can be potentially developed as for pig production. We aimed to identify are probiotic, good capacity of inhibiting pathogenic in intestine promising used substitutes antibiotics Potential probiotic strains were 15 fecal collected apparently healthy pigs, via 16S rDNA sequencing. The antimicrobial activity, tolerance bile salts, Caco-2 cell adhesiveness susceptibility isolates evaluated vitro, oral toxicity mice. One Lactiplantibacillus plantarum (BJR2), two Lacticaseibacillus casei (HJD TH2), one rhamnosus (MRS1), Enterococcus faecium (S-3 S-4-H) pigs underwent sequencing identification. L. BJR2 HJD exhibited broad-spectrum higher activity against indicator enteric pathogens, including Salmonella choleraesuis CVCC 2139, Escherichia coli (O147:K89) 199, (O141:K99) 223 (O139) 1496, among 6 tested strains. In addition, both low pH (pH 2.5 3.5) 0.30% had relatively strong carried no transferable resistant genes encoded by plasmid. safety trials, these α or β-hemolysis proved safe through tests It is concluded potential candidate their need further studied pigs.